Evaluating the p-FOXO1/FOXO1 Ratio: An Alternative Strategy for Endometrial Cancer Diagnosis

• 作者: Mohsen Korani Soudabeh Fallah
• 来源期刊: 临床医学国际期刊(英文)
• 学科: 医学
• 关键词: ENDOMETRIAL Cancer FOXO1 p-FOXO1 TRANSCRIPTION Factor
• 年，卷（期）: 2015,（3）
• 页码范围: 217-226
• 页数: 10页
• 分类号: R73

摘要

Background: The FOXO subfamily of Forkhead transcription factors plays a central role in pro-moting expression of proapoptotic and cell cycle regulatory genes. FOXO1 expression has an im-portant role in human endometrium homeostasis. Therefore, reduced FOXO1 protein and its inactivation by phosphorylation might, play a role in progression of human endometrial cancer. Methods: Current study was designed to investigate the changes of the FOXO1, phosphorylated-FOXO1 (p-FOX1) proteins levels and the p-FOXO1/FOXO1 ratio in 30 patients with endometrial cancer and 20 subjects with normal endometrium, surgically using excised human endometrial tissue specimens, quantitative real time PCR and western blot methods. Results: FOXO1 protein level in patients with endometrial cancer significantly reduced in comparison with control group (0.17 ± 0.15 vs. 1 ± 0.14;p