Simultaneous determination of <em>3</em>-chlorotyrosine and <em>3</em>-nitrotyrosine in human plasma by direct analysis in real time–tandem mass spectrometry

• 作者: Bin Wang Charles C.Liu Cheng Zha Jie Liao Yuqiao Song
• 来源期刊: 药学学报:英文版
• 学科: 医学
• 关键词: Simultaneous determination
• 年，卷（期）: 2015,（5）
• 页码范围: 482-486
• 页数: 5页
• 分类号: R969
• 语种: 中文

摘要

A novel method for the simultaneous determination of 3-nitrotyrosine(NT) and 3-chlorotyrosine(CT) in human plasma has been developed based on direct analysis in real time–tandem mass spectrometry(DART–MS/MS). Analysis was performed in the positive ionization mode using multiple reaction monitoring(MRM) of the ion transitions at m/z 216.2/170.1 for CT, m/z 227.2/181.1 for NT and m/z 230.2/184.2 for the internal standard, d~3-NT. The assay was linear in the ranges 0.5–100 μg/m L for CT and 4–100 μg/m L for NT with corresponding limits of detection of 0.2 and 2 μg/m L. Intra- and inter-day precisions and accuracies were respectively <15% and ±15%. Matrix effects were also evaluated. The method is potentially useful for high throughput analysis although sensitivity needs to be improved before it can be applied in clinical research.