Based on the effect of marine environment caused by sea reclamation and Technical Directives for Marine Ecological Capital Assessment(GB/T 28058-2011), the indexes of the loss of coastal ecosystem services led by sea reclamation were built. The lost value of sea reclamation was calculated using three methods, namely, economic evaluation, emergy analysis and biodiversity experiential formula. The three methods were applied in the new sea reclamation in Taxia, Fuding City, Fujian Province, and the total ecosystem lost value was 6.720 million CNY/year, 8.092 million CNY/year, and 1.333 million CNY/year, respectively. Compared with other relevant researches, this paper analyzed feasibility and applicability of the three methods, so as to include project external cost into the project economic profi t and loss analysis in the evaluation of environmental infl uence, and to provide technical support for the sustainable economic development of coastal zones.