基本信息来源于合作网站,原文需代理用户跳转至来源网站获取       
摘要:
AIM:To observe the expression of proline hydroxylase domain 2(PHD2) in the retina of diabetic rats and investigate the relationship between PHD2 and relevant intraocular vascular proliferation factors.· METHODS:Sixty male specific pathogen free(SPF)Sprague-Dawley(SD) rats were randomly divided into two groups:the diabetic group and the control group.The rats in the diabetic group were intraperitoneally injected with 60 mg/kg(0.60 mL/100 g) of streptozotocin to induce a diabetic rat model.The rats in the control group were injected with an equal volume of sodium citrate buffer solution by the same method.Hematoxylineosin(HE) staining and immumofluorescence(IF) method were adopted to observe the pathological changes of retinal tissues and the expression of PHD2,glial fibrillary acidic protein(GFAP),vascular endothelial growth factor(VEGF) by 8wk.RT-PCR method was applied to detect the expressions of mRNA of PHD2,VEGF and GFAP.The relationship between PHD2 and other vascular proliferation factors was analyzed.· RESULTS:HE staining showed that there was the retinal tissue edema in the diabetic group,and the arrangement was in disorder,and proliferation could be seen.IF staining:in the retina of normal rats,PHD2 was not expressed,GFAP and VEGF were mainly expressed in astrocytes;while in the diabetic rats,PHD2,GFAP and VEGF staining showed strong positivity in all retinal layers,mainly in neurogliocytes.PHD2 was co-expressed with VEGF and GFAP.The mRNA expression levels of PHD2,GFAP and VEGF in the diabetic group were obviously higher than that in the control group,respectively 1.83 times,1.75 times and 2.08 times.The difference had statistical significance(P<0.01).· CONCLUSION:The high expression of PHD2 in the retina of early-stage diabetic rats might result from secretion of neurogliocytes induced by local highconcentration blood glucose,thus promoting the expression of VEGF and GFAP.PHD2 plays an important role during the occurrence of diabetic retinopathy.
推荐文章
关于Domain半群与Domain半环
Domain半群
D-Clifford半群
Domain半环
Effects of a proline solution cover on the geochemical and mineralogical characteristics of high-sul
Proline
Coal gangue
Pollution control
Heavy metal fraction
Mineralogical characteristics
代数L-Domain的函数空间
L-domain
步函数
way-below关系
准连续Domain和稳定映射
准连续Domain
有界完备准连续Domain
稳定映射
内容分析
关键词云
关键词热度
相关文献总数  
(/次)
(/年)
文献信息
篇名 Express,on and effect of proline hydroxylase domain 2 in retina of diabetic rats
来源期刊 国际眼科杂志:英文版 学科 医学
关键词 proline hydroxylase domain 2 diabetic retinopathy retinal neovascularization
年,卷(期) 2016,(3) 所属期刊栏目
研究方向 页码范围 357-362
页数 6页 分类号 R77
字数 语种
DOI
五维指标
传播情况
(/次)
(/年)
引文网络
引文网络
二级参考文献  (0)
共引文献  (0)
参考文献  (23)
节点文献
引证文献  (0)
同被引文献  (0)
二级引证文献  (0)
2016(0)
  • 参考文献(0)
  • 二级参考文献(0)
  • 引证文献(0)
  • 二级引证文献(0)
研究主题发展历程
节点文献
proline
hydroxylase
domain
2
diabetic
retinopathy
retinal
neovascularization
研究起点
研究来源
研究分支
研究去脉
引文网络交叉学科
相关学者/机构
期刊影响力
国际眼科杂志:英文版
月刊
2222-3959
西安市友谊东路269号
出版文献量(篇)
2720
总下载数(次)
2
总被引数(次)
0
论文1v1指导