4-70 Efficiency of Dyes in STORM Influences the Result of Dual Color Experiment

• 作者: Chen Hao Du Guanghua Guo Jinlong Li Xiaoyue Li Yaning Liu Wenjing Wei Junzhe Wu Ruqun Zhao Jing
• 来源期刊: 中国科学院近代物理研究所和兰州重离子研究装置年报：英文版
• 学科: 物理学
• 年，卷（期）: 2016,（1）
• 页码范围: 197-197
• 页数: 1页
• 分类号: O4

摘要

Stochastic Optical Reconstruction Microscopy(STORM)is one of the super-resolution microscopy,which pro-vides resolutions down to a few tens of nanometers by exploiting the cycling of dyes between uorescent and nonuorescent states to obtain a sparse population of emitters and precisely localizing them individually[1].The key material is the uorescent dyes are photo-switchable thousands of times before they turn quenched completely.Fluorescent proteins and organic dyes are the two main kinds of uorescent dyes in the experiment[2].The former are widely used in the living cell,the latter are used in the xed cells in the form of single dye or dye pair[3].