Effects of dexamethasone and HA1077 on actin cytoskeleton and β-catenin in cultured human trabecular meshwork cells

• 作者: Bo Gong Chun-Tao Lei Jie Peng Qian Luo Xiao-Yun Feng Yi-Lian Cheng Zheng-Zheng Wu Zi-Meng Ye
• 来源期刊: 国际眼科杂志：英文版
• 学科: 医学
• 年，卷（期）: 2016,（10）
• 页码范围: 1376-1380
• 页数: 5页
• 分类号: R77

摘要

AIM:To investigate the effects of dexamethasone(DEX) and 1-(5-isoquinolinesulfonyl)-homopiperazine(HA1077) on actin cytoskeleton and β-catenin in cultured human trabecular meshwork(HTM) cells.METHODS: The HTM cells were separated from human eyeball and cultured in vitro.They were divided into control group,DEX(1×10~(-6)mol/L) group,HA1077(3×10~(-5)mol/L)group,and DEX(1×10~(-6)mol/L) and HA1077(3×10~(-5)mol/L)group.Actin cytoskeleton and β-catenin in HTM cells of the four groups were examined by immunofluorescence and Western blot analyses.RESULTS: In DEX group,there were reorganization of actin cytoskeleton and formation of cross linked actin networks(CLANs),which were partially reversed in DEX and HA1077 group.DEX treatment also induced an increased expression of β-catenin,which was obviously reduced in DEX and HA1077 group.Meanwhile,the cultured HTM cells in HA1077 group had lower expression of β-catenin than that in the control group. CONCLUSION: Our results show that HA1077 can reverse the changes of actin organization and expression of β-catenin induced by DEX in cultured HTM cells,suggesting that HA1077 may play an important role in increasing outflow and reducing intraocular pressure.