The involvement of proline-rich protein Mus musculus predicted gene 4736 in ocular surface functions

• 作者: Feng Zhang Sheng-Wei Ren Xia Qi Yi-Qiang Wang
• 来源期刊: 国际眼科杂志：英文版
• 学科: 医学
• 关键词: proline-richprotein Musmusculus predicted GENE 4736 OCULAR surface PSEUDOMONAS AERUGINOSA KERATITIS
• 年，卷（期）: 2016,（8）
• 页码范围: 1121-1126
• 页数: 0页
• 分类号: R772.21

摘要

AIM: To research the two homologous predicted proline-rich protein genes, Mus musculus predicted gene 4736(MP4) and proline-rich protein Bst NI subfamily1(Prb1) which were significantly upregulated in cultured corneal organs when encountering fungal pathogen preparations. This study was to confirm the expression and potential functions of these two genes in ocular surface.METHODS: A Pseudomonas aeruginosa keratitis model was established in Balb/c mice. One day post infection, m RNA level of MP4 was measured using real-time polymerase chain reaction(PCR), and MP4 protein detected by immunohistochemistry(IHC) or Western blot using a customized polyclonal anti-MP4 antibody preparation. Lacrimal glands from normal mice were also subjected to IHC staining for MP4. An online bioinformatics program, Bio GPS, was utilized to screen public data to determine other potential locations of MP4.RESULTS: One day after keratitis induction, MP4 was upregulated in the corneas at both m RNA level as measured using real-time PCR and protein levels as measured using Western blot and IHC. Bio GPS analysis of public data suggested that the MP4 gene was most abundantly expressed in the lacrimal glands, and IHC revealed that normal murine lacrimal glands were positive for MP4 staining.CONCLUSION: MP4 and Prb1 are closely related with the physiology and pathological processes of the ocular surface. Considering the significance of ocular surface abnormalities like dry eye, we propose that MP4 and Prb1 contribute to homeostasis of ocular surface, and deserve more extensive functional and disease correlation studies.