BFGF induces proliferation and migration of mouse bone mesenchymal stem cells

• 作者: Rui Cao Xin-Yuan Luan Xiong-Zhi Wu Xiu-Li Ding Xue Yang
• 来源期刊: 肿瘤微环境研究
• 学科: 医学
• 关键词: BFGF BONE MARROW MESENCHYMAL stem cells PROLIFERATION MIGRATION
• 年，卷（期）: 2019,（1）
• 页码范围: 2-7
• 页数: 6页
• 分类号: R73

摘要

In order to investigate the effects of basic fibroblast growth factor(bFGF)on the proliferation and migration of bone mesenchymal stem cells(BMSCs).Mouse BMSCs were isolated by adherent culture in vitro.The surface markers CD29 and CD44 were detected by flow cytometry.The effect of 10 ng/ml bFGF on the proliferation of BMSCs was detected by trypan blue counting method for 48 hours.Scratch test and transwell assay were used to detect the effect of 10 ng/ml bFGF on cell migration of BMSCs.Mouse BMSCs were successfully isolated and purified.The surface markers CD29 and CD44 was positive.Trypan blue staining showed that 10 ng/ml bFGF significantly promoted the proliferation of BMSCs(P<0.05);the scratch test showed that after adding 10 ng/ml bFGF,the migration distance of the experimental group was significantly longer than that of the control group.And the mobility ratios at 6h and 12h were 1.57±0.31(P<0.05)and 2.07±0.95(P<0.05).Transwell results showed that the number of cell migration in the experimental group was significantly higher than that in the control group after 12 h of 10 ng/ml bFGF(P<0.01).Result confirmed that bFGF induces proliferation and migration of mouse BMSCs.