Cost-reduction strategies in massive genomics experiments

• 作者: Chenchen Ruan Haichao Li Hongan Long Jiao Pan Kun Wu Yujin Wang
• 来源期刊: 海洋生命科学与技术(英文)
• 学科: 文学
• 关键词: 3D model designing and PRINTING GENOME SEQUENCING Lab-made tools Library construction MINIATURIZATION
• 年，卷（期）: 2019,（1）
• 页码范围: 15-21
• 页数: 7页
• 分类号: H31

摘要

Many modern biology studies require deep, whole-genome sequencing of hundreds to thousands of samples. Although persamplecosts have dramatically decreased, the total budget for such massive genome sequencing constitutes a significantbarrier for poorly funded labs. The costly lab tools required for genomics experiments further hinder such studies. Here, weshare two strategies for extensively reducing the costs of massive genomics experiments, including miniaturization of theNEBNext Ultra II FS DNA Library Prep Kit for Illumina (reducing the per-sample total costs to ~ 1/6 of that charged byservice providers) and in-lab 3D model-designing of genomics tools. These strategies not only dramatically release fundingpressure for labs, but also provide students with additional training in hands-on genomics and 3D-model-designing skills,demonstrating the high potential for their application in genomics experiments and science education.