ID7 Isolated from Bauhinia variegata Stem Inhibits Tumor Progression and Metastatic Mechanisms of Triple Negative Breast Cancer in Vivo

• 作者: Bruno Oliveira Fabrício P.Batista Fernanda E.Pinto Hélio B.Santos Izabela Natalia Faria Gomes Kamilla Monteiro dos Santos Maria Luiza Vilela Oliva Rafael Cesar Russo Chagas Ralph G.Thomé Renato JoséSilva Oliveira Rosy Iara Maciel de Azambuja Ribeiro Rui Manuel Reis Wanderson Romao
• 来源期刊: 药剂与药理学:英文版
• 学科: 医学
• 关键词: 4T1 cells MDA-MB-231 MEDICINAL plants KAEMPFEROL GELATINASES apoptosis cell viabilty in vivo
• 年，卷（期）: 2019,（7）
• 页码范围: 368-384
• 页数: 17页
• 分类号: R

摘要

Background:The breast cancer has been the most common form of cancer among women.The triple negative subtype represents 20%of all breast cancer cases in the world and is standing out by affecting young women and being aggressive.The main cause of death of patients with cancer is due to metastasis,which can reach the liver and lungs.Objective:The activities of ID7 fraction of the stems of Bauhinia variegata L.on breast cancer,lung metastasis and liver inflammatory process were evaluated.Method:ID7 was characterized by mass-spectrometry.The viability of murine mammary cells(4T1)treated with ID7 was assessed by MTT,trypan blue and fluorescence assay and viability of BT-20,MDA-MB-231 and MCF-7 human breast cancer tumor lines by MTS.The cell migration,invasion using matrigel and adhesion were performed.The expression of cell death proteins was quanitified by western blot and the gelatinases by zimogram.The ID7 activity of the tumor(4T1)and metastatic progession in vivo was evaluated.Results:ID7 reduced the 4T1 and MDA-MB-231viability and increased the late apoptosis,inhibited the 4T1 migration and invasion,increased the 4T1 adhesion and decreased the secreted active gelatinases.ID7 also increased the expression of PARP,caspase-7 and caspase-8,RIP and TNF-R1.In vivo,the ID7 decreased the volume and weight of the tumors and decreased lung metastasis and inflammation in the liver.The characterization showed mainly the presence of oleic acid,myricetin,quercetin and kaempferol in ID7.Conclusion:Thus,it was found that ID7 fraction exhibits selective antitumor and on the mechanisms of breast cancer metastasis activity,preventing lung metastasis and inflammation in the liver.It is suggested that fatty acids and flavonoids are correlated with such activities.