Phosphorylation of AMMECR1 at Serine16 Is Not Essential for Its Nuclear Localization

• 作者: Chengfeng Cai Huamin Zhou Meng Xu Yongquan Su
• 来源期刊: 生物科学与医学(英文)
• 学科: 物理学
• 关键词: AMMECR1 TRANSCRIPTION PHOSPHORYLATION NUCLEAR LOCALIZATION
• 年，卷（期）: 2019,（11）
• 页码范围: 146-153
• 页数: 8页
• 分类号: O57

摘要

AMMECR1 is a critical gene in the pathogenesis of AMME syndrome. But, little is known about how AMMECR1 is regulated. Here, we showed that many human cell lines expressed AMMECR1. With Phos-tag SDS PAGE analysis, we demonstrated that AMMECR1 was constitutively phosphorylated at Ser16. AMMECR1 was localized in the nucleus. Mutation of Ser16 to alanine did not affect its nuclear localization. The homologs of AMMECR1, PAC688.03c and MTH857, were also nuclear proteins, but they were not phosphorylated when tested in HeLa cells. Therefore, AMMECR1 and its homologs might have atypical nuclear localization sequences.