Inhibition of Obtusifolin on retinal pigment epithelial cell growth under hypoxia

• 作者: Li-Fei Wang Lu Lu Shan-Yu Li Sheng-Juan Zhang Xin Jia Xin Wang Ya-Lin Li Yan-Hui Wang Yan-Xia Shang Yun-Huan Li Zhong-Yang Yan
• 来源期刊: 国际眼科杂志:英文版
• 学科: 医学
• 关键词: RETINAL PIGMENT EPITHELIAL cells Obtusifolin vascular ENDOTHELIAL GROWTH factor HYPOXIA
• 年，卷（期）: gjykzzywb_2019,（10）
• 页码范围: 1539-1547
• 页数: 9页
• 分类号: R774

摘要

AIM: To explore the effect of Obtusifolin on retinal pigment epithelial cell growth under hypoxia.METHODS: In vitro chemical hypoxia model of ARPE-19 cells was established using cobalt chloride(CoCl2). Cell viability was tested by cell counting kit-8(CCK-8) assay. Western blot and real-time quantitative polymerase chain reaction were applied to detect proteins and mRNAs respectively. Flow cytometry was used to examine the cell cycle. Secretion of vascular endothelial growth factor(VEGF) was tested by using enzyme linked immunosorbent assay(ELISA).RESULTS: Under the chemical hypoxia model established by CoCl2, hypoxia inducible factor-1α(HIF-1α) mRNA and protein levels was up-regulated. Cell viability was increased and the proportion of S phase was higher. Obtusifolin could reduce cell viability under hypoxic conditions and arrest cells in G1 phase. Obtusifolin reduced the expression of Cyclin D1 and proliferating cell nuclear antigen(PCNA) in the hypoxic environment and increased the expression of p53 and p21. The levels of VEGF, VEGFR2 and eNOS proteins and mRNA were significantly increased under hypoxia while Obtusifolin inhibited the increasing.CONCLUSION: Obtusifolin can inhibit cell growth under hypoxic conditions and down-regulate HIF-1/VEGF/eNOS secretions in ARPE-19 cells.