Optimization of Coenzyme Q10Production Procedure from Genetic Engineered Rhodobacter sphaeroides Overexpressing UbiG

• 作者: Jiamin ZHANG Lili JI Wei WANG Xinning XU Zhiping ZHAO Ziyi YE
• 来源期刊: 农业生物技术:英文版
• 学科: 工学
• 关键词: COQ 10 RHODOBACTER SPHAEROIDES GENETIC engineering OPTIMIZATION FERMENTATION
• 年，卷（期）: 2020,（1）
• 页码范围: 6-8
• 页数: 3页
• 分类号: TQ9

摘要

In the present study, single factors including fermentation temperature, inoculate amount, fermentation duration, and ratio of fermentation medium volume to total flask volume(dissolved oxygen tension) were optimized for enhancing the production of coenzyme Q10 from genetic engineered Rhodobacter sphaeroides overexpressing UbiG. The experimental results suggested that optimal single factors were: inoculate amount 2%, fermentation temperature 30 ℃, fermentation duration 48 h, and ratio of fermentation medium volume to total flask volume 80%. The present study will promote the large scale production of CoQ10 from microorganisms.