Association of ACE Gene with Metabolic Syndrome, Hypertension and Alzheimer Disease by RFLP-PCR

• 作者: Stanislav Alexandra Alina
• 来源期刊: 化学与化工:英文版
• 学科: 医学
• 关键词: ACE gene metabolic syndrome HYPERTENSION Alzheimer disease
• 年，卷（期）: 2020,（4）
• 页码范围: 105-112
• 页数: 8页
• 分类号: R54

摘要

ACE gene is associated with multifactorial diseases:metabolic syndrome,obesity,diabetes mellitus,hypertension,stroke,myocardial infarction,ageing,Alzheimer disease,acute pulmonary failure and COVID-19 infections.The purpose of this study is to test the association between the II,ID and DD polymorphisms of angiotensin-converting enzyme(ACE)gene,and metabolic syndrome,hypertension(HBP)and Alzheimer disease(AD),based on clinical data and biochemical laboratory investigations conducted on inpatients,applying the RFLP-PCR technique.The genotyping of ACE gene was carried out by RFLP-PCR on the basis of DNA isolated from total blood in 144 subjects selected at Giurgiu County Emergency Hospital.The results were statistically processed by the Hardy-Weinberg equilibrium,Odds Ratio,VMD,StatsDirect and PyElph software.The II,ID and DD polymorphisms of ACE gene identified by the RFLP-PCR present a high risk of developing the metabolic syndrome in the MS,hypertension and Alzheimer disease groups.