Objective To analyze the etiology of food poisoning, provide data support for food poisoning treatment, and protect people's health. Methods RT-PCR was used to screen suspicious food and vomit. An enzyme-linked immunofluorescence assay ( ELFA) was used to detect Staphylococcus aureus enterotoxin. RT-PCR was used to detect enterotoxin genotypes. An automatic drug sensitivity reading system was used for drug resistance analysis and mass spectrometry identification. Re-sults Staphylococcus aureus were isolated from 5 leftovers and 2 vomitus samples. The enterotoxin types of 7 strains were sed and seen;the drug sensitivity test showed that 7 strains were resistant to penicillin, and 6 strains were inducible clinda-mycin resistant. Conclusion This is a food poisoning event caused by Staphylococcus aureus, which produces sed and sees enterotoxins. The isolated strains have different degrees of drug resistance.