A novel microfluidic flow-cytometry for counting numbers of single-cell β-actins

• 作者: Beiyuan Fan Deyong Chen Hongyu Yang Jian Chen Junbo Wang Lixing Liu Shuang Zhang
• 来源期刊: 纳米技术与精密工程（英文）
• 年，卷（期）: 2020,（3）
• 页码范围: 156-161
• 页数: 6页
• 语种: 英文

摘要

As a house keeping protein with stable expressions,β-actin is used as a loading control in normalization of west-ern blotting. However, the actual numbers ofβ-actins at the single-cell level remain elusive. Based on a home-developed flow cytometry, single-cell numbers ofβ-actin from 8 cell types (subtypes) and 2 tumour patient sam-ples were quantified as 9.62 ± 4.29 × 105 (A549, Ncell=14,242), 6.46 ± 3.34 × 105 (Hep G2, Ncell=35,932), 1.58 ± 0.90 × 106 (MCF 10A, Ncell=16,650), 1.08 ± 0.48 × 106 (HeLa, Ncell=26,151), 7.60 ± 4.34 × 105 (PC3, Ncell=11,922), 1.10 ± 0.72 × 106 (SACC-83, Ncell=13,616), 8.58 ± 4.54 × 105 (CAL 27, Ncell=7271), 9.00 ± 4.69 × 105 (CAL 27-LN2, Ncell=6222), 8.26 ± 4.48 × 105 (Oral Tumour Patient I, Ncell=359), and 8.19 ± 5.12 × 105 (Oral Tumour Patient Ⅱ, Ncell=175), and were analyzed by statistical approaches including one-way analysis of variance, neural network based pattern recognition and Bayesian estimation, with varied ex-pressions ofβ-actins among different cell types located. The dataset reported in this study may serve as a refer-ence in future studies of quantitative protein analysis.