Cryo-EM structure of a type I-F CRISPR RNA guided surveillance complex bound to transposition protein TniQ
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摘要:
Dear Editor,
CRISPR-Cas systems provide adaptive immunity in prokaryotes through RNA-guided cleavage of mobile genetic elements and have been harnessed as powerful genome editing tools.A programmable guide RNA and the effector Cas protein are delivered to mammalian cells to create double strand breaks on the target sequence in the genome,triggering endogenous DNA repair pathways that enable genetic changes.However,recent studies revealed transposon-associated CRISPR-Cas systems that are inactive in target cleavage but direct transposases for RNA-guided DNA transposition.1-4 Those findings open a new paradigm for precise DNA insertion independent of DNA repair pathways.