Cloning and Expression Analysis of Heat Shock Protein Gene DpHsp70 in Dahlia

• 作者: Chuanle ZHAO Hua GUAN Jianzhong TAN Lan LUO Siyu ZHOU Siyu ZHU
• 来源期刊: 农业生物技术:英文版
• 学科: 医学
• 关键词: DAHLIA High temperature stress Salicylic acid(SA) Heat shock protein Gene expression
• 年，卷（期）: 2020,（4）
• 页码范围: 1-3
• 页数: 3页
• 分类号: R73

摘要

[Objectives]This study was conducted to investigate the relationship between HSPs and the response of high temperature stress.[Methods]Molecular biological techniques were applied to clone and analyze the gene sequence of DpHsp70 gene.The changes in the expression of DpHsp70 gene under high temperature stress and exogenous salicylic acid(SA)were observed and further analyzed by qRT PCR.[Results]The coding region of the Dahlia DpHsp70 gene was 705 bp,encoding 234 amino acid residues(GenBank accession number:MH102288).Aligned with Compositae plants,the Dahlia DpHsp70 gene shared more than 83%homology in gene sequence while 99%-100%homology in amino acid sequence.Under the 35℃high temperature stress,the expression of DpHsp70 gene in Dahlia petals significantly increased.Meanwhile,the expression of DpHsp70 gene further increased under SA at 35℃temperature,which was significantly higher than those of the control group and the 35℃high temperature stress treatment group.It was demonstrated that the Hsp70 gene in Compositae is with highly conservative property and its expression could be up-regulated in response to high temperature stress.It can also be concluded that applying exogenous SA can improve the high temperature resistance of Dahlia.[Conclusions]This study provides a new experimental basis for elucidating the physiological function and mechanism of Dahlia in response to high temperature stress.