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The aim of the study was to analyze the input of cytosolic and oxidative pathways of energy metabolism in ATP production of cultured cells by using FLIM and routine biochemical techniques. Fluorescent imaging of endogenous cofactors NADH and FAD demonstrated a more pronounced oxidative redox status of fibroblasts compared to tumor cells and significant differences in metabolic processes in which FAD is involved. Analysis of glucose and lactate content and absorption showed that tumor cells not only absorb glucose more intensively from the environment, but also use it more intensively during anaerobic glycolysis. Lower energy efficiency of glycolysis and FAD oxidative path and greater energy consumption is the reason for the lower concentration of ATP in tumor cells. Presumably, the prevalence of glycolytic metabolism in tumor cells could be largely determined by their hypoxic reprogramming through the PI3K/AKT/mTOR signal pathway. The results of the study suggest that correlation between intracellular consumption of glucose and cytosolic concentration of NADH may contribute to the characteristic of energy metabolism state of cultured cells and serve as the biosensor of malignant cell transformation.
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篇名 A New Glance on Energy Metabolism Investigation in Cultured Cells
来源期刊 生物科学与医学(英文) 学科 医学
关键词 Metabolic Imaging GLYCOLYSIS ATP Tumor Cells of HeLa Kyoto
年,卷(期) 2020,(8) 所属期刊栏目
研究方向 页码范围 45-55
页数 11页 分类号 R73
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Metabolic
Imaging
GLYCOLYSIS
ATP
Tumor
Cells
of
HeLa
Kyoto
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生物科学与医学(英文)
月刊
2327-5081
武汉市江夏区汤逊湖北路38号光谷总部空间
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721
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