Tissue Culture and Rapid Propagation Techniques of New Variety in Cymbidium hybridum

• 作者: Jinqiao PENG Liyuan YANG Rongjiao DONG Shaobo ZHANG Xiangbei LI Xiaofei YANG Xinglong WU
• 来源期刊: 农业生物技术:英文版
• 学科: 农学
• 关键词: Cymbidium hybridum Tissue Culture PROTOCORM Proliferation and differentiation
• 年，卷（期）: 2020,（3）
• 页码范围: 8-11
• 页数: 4页
• 分类号: S68

摘要

[Objectives]This study was conducted to select media suitable for proliferation,differentiation and rooting of Cymbidium hybridum"Huangjinjia".[Methods]The lateral buds and protocorms of the new variety C.hybridum"Huangjinjia"were used as materials to explore the effects of different concentrations of 6-BA and NAA on protocorm proliferation and rooting.[Results]The optimal medium for protocorm propagation was 1/2 MS+6-BA 1.0 mg/L+NAA0.5 mg/L+potato 50 g/L+sucrose 20 g/L,in which the protocorms multiplied easily and grew rapidly.The optimal medium for inducing adventitious buds was1/2 MS+6-BA 1.5 mg/L+NAA 0.3 mg/L+sucrose 30 g/L+banana 25 g/L+apple 25 g/L+activated carbon 1.0 g/L,in which the induction rate of adventitious buds reached 335%.The optimal medium for rooting was 1/2 MS+NAA 0.5 mg/L+sucrose 25 g/L+banana 75 g/L+apple 25 g/L+activated carbon1.0 g/L,in which the average root length was 3.0 cm,the average number of roots was 2.6,and plantlets had green leaves,thick roots and suitable plant height.[Conclusions]This study provides a theoretical basis and reference for the establishment of a rapid propagation system using lateral buds.