Construction and Identification of Intestine-specific Expression Vector of Xylanase Gene(XynB)

• 作者: Hui KE Sheng GAO Yulong WANG Zhe CHEN
• 来源期刊: 农业生物技术:英文版
• 学科: 医学
• 关键词: XYLANASE Specific expression vector Resistin-likeβgene WB detection RT-PCR detection
• 年，卷（期）: 2020,（5）
• 页码范围: 1-4
• 页数: 8页
• 分类号: R73

摘要

The Aspergillus niger XynB gene and core promoter region of porcine RELMβgene were cloned into pcDNA3.1(-),and an intestine-specific expression vector pcDNA3.1-RELMβ-XynB-Myc-GFP carrying green fluorescence and Myc double tags was constructed.The vector was transfected into human colon cancer cells(HT29)and human liver cancer cells(Bel7402)using liposomes.Fluorescence microscopy revealed that the vector could specifically express green fluorescent protein(GFP)in HT29 cells.RT-PCR and Western Blot were performed on the HT29 cells transfected with the expression vector,and the results showed that the XynB gene was normally transcribed in HT29 cells,and the target protein expression was detected in the cells.