Elevated miR-10a-5p facilitates cell cycle and restrains adipogenic differentiation via targeting Map2k6 and Fasn,respectively

• 来源期刊: 生物化学与生物物理学报（英文版）
• 年，卷（期）: 2020,（11）
• 所属期刊栏目: Original Articles
• 页码范围: 1227-1235
• 页数: 9页
• 语种: 英文

摘要

miRNAs are a small class of noncoding RNAs that perform biological functions by regulating the stability or translation of target genes in various biological processes.This study illustrated the role of miR-10a-5p,which is relatively enriched in adipose tissues,using primary mouse preadipocytes as model.With elevated miR-10a-5p expression,the proliferative ability of mouse preadipocytes was significantly enhanced,indicated by increased EdU+ cells and Gi/S transition,accompanied by upregulated Cyclin B,Cyclin D and PCNA and downregulated p21 and p27.Meanwhile,the adi-pogenic differentiation was significantly attenuated by elevated miR-10a-5p,supported by Oil Red O staining and suppressed PPARy and aP2 expression.Furthermore,Map2k6 and Fasn were pre-dicted to be the target genes of miR-10a-5p in silico,and dual luciferase reporter assay confirmed the direct targeting effects.Western blot analysis results showed that miR-10a-5p specially reduced Map2k6 expression at the proliferative stage without affecting Fasn expression,while significantly restrained Fasn expression with unchanged Map2k6 expression during adipogenic differentiation.Taken together,these results revealed a potential role of miR-10a-5p in adipogenesis and in the treatment of obesity.