NIR-Ⅱ probe modified by poly(L-lysine) with efficient ovalbumin delivery for dendritic cell tracking

• 来源期刊: 中国科学：化学（英文版）
• 年，卷（期）: 2020,（9）
• 所属期刊栏目: ARTICLES
• 页码范围: 1272-1280
• 页数: 9页
• 语种: 英文

摘要

Dendritic cell (DC) vaccine is an effective strategy for cancer immunotherapy by carrying antigen into DCs and migrating these DCs to drain lymph nodes after inoculation.In this article,second near-infrared window (NIR-Ⅱ) fluorescent nanoparticles have been used to uptake antigen and activate DCs.Ovalbumin (OVA),an antigen for immunization,can be loaded on the surface of these NIR-Ⅱ fluorescent nanoparticles via electrostatic interaction by virtue of their functionalized poly(L-lysine) (PLL),which exhibits biocompatibility and strong selective interaction with OVA.In addition,these antigen-loaded complexes can efficiently be engulfed by immature DCs to induce DC maturation and cytokine secretion.After subcutaneous injection,highly sensitive NIR-Ⅱ fluorescence signal from nanoparticles indicates that nanoparticle-labeled DCs can successfully migrate into lymph nodes in vivo,showing great promise in immunotherapy against cancer.