Oxidative stress and mitochondrial dysfunction of retinal ganglion cells injury exposures in long-term blue light

• 作者: Chen Huang Ke-Xin Guo Min-Shu Wang Pei Zhang Wei Wang Ying Li Zi-Yuan Liu
• 来源期刊: 国际眼科杂志:英文版
• 学科: 医学
• 关键词: blue light retinal ganglion cell oxidative stress mitochondria metabolism mitochondria abnormalities mitogen-activated protein kinases AKT
• 年，卷（期）: 2020,（12）
• 页码范围: 1854-1863
• 页数: 10页
• 分类号: R774.1

摘要

AIM:To investigate the phototoxic effect of long-term excessive narrow-band blue light in staurosporine-induced differentiated retinal ganglion cells-5(SSRGC-5).METHODS:SSRGC-5 cells were divided into two groups,blue light group(BL group)and control group.Cell viability was assessed by using CCK-8 assay.Metabolic profile analysis was performed by using Seahorse extracellular flux analyzer.Mitochondria ultrastructure were studied via transmission electron microscope(TEM).Mitochondria contents and oxidative stress was evaluated by flow cytometry.Western blotting was performed to monitor the changes in mitogen-activated protein kinases(MAPK)pathway and PI3 K/AKT pathway.RESULTS:Blue light caused morphological changes of SSRGC-5 cells.The cell viability was significantly decreased from 3 h in BL group.Intercellular ROS and mitochondrial superoxide levels were increased following blue light exposure.Metabolic profiling identified blue light induced SSRGC-5 cells to have severely compromised mitochondrial function.This was accompanied by impaired mitochondrial ultrastructure and remodeling,increased expression of the mitochondrial related proteins,and increased glycolysis as compensation.Moreover,the results showed that blue light induced higher expression of p-p38,p38,p-JNK,p-ERK,p-cJun,c-Jun,and p-AKT.CONCLUSION:These findings indicate that excessive narrow-band blue light induces oxidative stress and mitochondrial metabolic remodeling dysregulate in SSRGC-5 cells.Activated MAPK and AKT signaling pathways are involved in this process.