Optimization of Cowpea Dry Grain Yield through the Stimulation of the RNA Synthetic Activity of NADH-Glutamate Dehydrogenase

• 作者: Godson O. Osuji Paul M. Johnson Wenceslaus C. Madu
• 来源期刊: 美国植物学期刊(英文)
• 学科: 化学
• 关键词: Stoichiometric Mineral Salt Mixes Glutamate Dehydrogenase Cycle Photosynthesis NODULATION VICILIN Double Grain Yields Food Security
• 年，卷（期）: mgzwxqkyw_2021,（1）
• 页码范围: 71-103
• 页数: 33页
• 分类号: O62

摘要

Several potentially practical biochemical processes in plant systems still remain hidden, especially the NADH-glutamate dehydrogenase (GDH) synthesis of nongenetic code-based RNA that optimizes crop nutritious yield by degrading superfluous genetic code-based RNA. In continued characterization of the biochemistry of cowpea grain yield, GDH was purified by electrophoresis from seeds of cowpea treated with solutions of stoichiometric mixes of mineral salts. The GDH was made to synthesize RNAs in the amination (α-KG/NADH/</span><span><span></span><span style="font-family:""><span style="font-family:Verdana;">) and then in the deamination (L-Glu/NAD</span>^{<span style="font-family:Verdana;">+</span>}<span style="font-family:Verdana;">) direction. The initial product RNAs were captured and sequenced. The grand challenge was to discover the specific molecular roles of the redox enzyme in the optimization of cowpea grain yields. In the amination direction, the GDH hexamers synthesized plus-RNA, but in the deamination direction</span></span><span style="font-family:Verdana;">,</span><span style="font-family:""><span style="font-family:Verdana;"> they synthesized minus-RNA. The plus-RNAs and minus-RNAs were homologous to about the same numbers of different mRNAs encoding the key enzymes that regulate photosynthesis;saccharide biochemistry and glycolysis;phenylpropanoid biosynthesis;nodulation nitrogen fixing processes;dehydrin drought and glutathione environmental stress resistance processes;purine, pyrimidine, DNA, RNA and essential amino acid biosynthesis;storage protein vicilin accumulation;isoflavone earliness of cowpea maturity;peroxidase synthesis of lignin and sequestration of CO</span>_{<span style="font-family:Verdana;">2</span>}<span style="font-family:Verdana;"> to enrich soil organic carbon contents;triglyceride physiology in the biosynthesis of bioactive compounds that render cowpea resistant to insects and fungi;etc</span></span><span style="font-family:Verdana;">.</span><span style="font-f