Preparation and Insecticidal Activity of Sea Anemone Peptide AP-GI from Aiptasia pallida

• 作者: Jinxing FU Yanling LIAO Xuefeidan LIU Bingmiao GAO
• 来源期刊: 农业生物技术:英文版
• 学科: 化学
• 关键词: Aiptasia pallida Sea anemone peptide Solid phase peptide synthesis Oxidative folding Insecticidal activity
• 年，卷（期）: nyswjsywb_2021,（1）
• 页码范围: 49-52
• 页数: 4页
• 分类号: O62

摘要

[Objectives] This study was conducted to synthesize sea anemone peptide Ap-GI and investigate its insecticidal activity. [Methods] The linear peptide Ap-GI was synthesized by solid phase peptide synthesis(SPPS), and the linear peptide was subjected to the two-step oxidative folding, mass spectrometry identification and high performance liquid chromatography(HPLC) purification. Then, the MTT method and insect injection method were used to study its insecticidal activity. [Results] The synthesized sea anemone peptide had a purity of 95%. The test results of the MTT method showed that the peptide Ap-GI had the activity of inhibiting the growth of insect cells sf9 with the median effective dose of 0.7 nM;and the test results of the injection method on yellow mealworms showed that the peptide Ap-GI had high insecticidal activity, and the median lethal dose was 16.9 nM. [Conclusions] The sea anemone peptide Ap-GI from Aiptasia pallida has a good inhibitory effect on the growth of insect cells and high-efficiency insecticidal activity, which can lay a foundation for the development of new, safe and efficient peptide biological insecticides.