miR-146a-5p affects inflammation response of trophoblast by inhibiting TRAF6/NF-кB signaling pathway

• 作者: Fang-Rong Chen Dong-Cai Wu Xiao-Ju Chen
• 来源期刊: 海南医科大学学报（英文版）
• 年，卷（期）: 2021,（6）
• 所属期刊栏目: Original article
• 页码范围: 10-14
• 页数: 5页
• 语种: 英文

摘要

Objective: To investigate the association of Micro-rna (miR)-146a-5p expression with preeclampsia, and further explore the potential mechanism involved. Methods: Compared with the blank control group, the expressions of miR-146a-5p and TRAF6 were detected in lipopolysaccharide (LPS)-induced JEG-3 cells. Chorionic carcinoma cell JEG-3 in vitro culture are divided into control, miR-146a-5p mimic + lipopolysaccharide (lps), miR-146a-5p mimic and miR-146a-5p inhibitor groups. qRT-PCR analysis were used to detect the mRNA of miR-146a-5p,IL-1β,IL-6, IL-8 and TNF-α. Western blot assays were carried out to determine the protein expression of TRAF6/NF-кB pathway related proteins. Results: 1.miR-146a expression in miR-146a mimic group were significantly higher than the other three groups(P <0.05). 2.Compared with the control group, the expression level of miR-146a-5p in JEG-3 cells induced by LPS was significantly increased, and the expression level of TRAF6 was significantly reduced (P <0.05). 3.Compared with the control group, the mRNA expression levels of IL-1β, IL-6, IL-8, and TNF-αdecreased significantly after using miR-146a mimic (P <0.05). After adding miR-146a inhibitor, the mRNA expression levels of IL-1β, IL-6, IL-8, and TNF-α were significantly increased (P <0.05). However, compared with the mimic + LPS group, the difference was not statistically significant (all P > 0.05). The results of Western Blot showed that the expression of TRAF6 and NF-κB protein in JEG-3 cells decreased significantly after adding miR-146a mimic and increased after adding miR-146a inhibitor. Conclusion: MiR-146-5p can affect the inflammation response of Maternal-fetal interface by inhibiting TRAF6 / NF-кB signaling pathway in preeclampsia.