Effects of controlled superovulation on the expression of HOXA10, integrin αvβ3 and LIF in mice during peri-implantation

• 作者: Lei Zhang Jie Liu Ying Zhang Fang Wang Chang-Lei Li Ai-Ni Fu Jun You Shu-Xiu Zhu
• 来源期刊: 海南医科大学学报（英文版）
• 年，卷（期）: 2021,（8）
• 所属期刊栏目: Original article
• 页码范围: 7-11
• 页数: 5页
• 语种: 英文

摘要

Objective: To observe the expression of endometrial homologous box gene A10 (HOXA10), Integrin αvβ3 and leukemia suppressor factor (LIF) in peri-implantation mice, and to investigate the effect of controlled superovulation (COH) on endometrial receptivity in mice. Methods: After COH model preparation and blastocyst transplantation, kunming pure-bred mature mice were randomly divided into natural blastocyst + natural pseudocyst group, COH blastocyst + natural pseudocyst group, natural blastocyst +COH pseudocyst group, and COH blastocyst +COH pseudocyst group.The pregnancy rate and average number of beds in each group were observed on day 6 of conception. Western blot and RT-PCR were used to detect the expression of HOXA10, Integrin αvβ3 , LIF protein and mRNA in the endometria of mice on day 5 of conception. Results: The pregnancy rate and average number of beds in the COH group were lower than those in the natural receptor group.The expression of HOXA10, Integrin αvβ3 and LIF in endometrium was significantly lower than that of the natural receptor mice (P< 0.01). Conclusion: Superovulation drugs down-regulate the expression of HOXA10,Integrin αvβ3 and LIF in endometrium during the implantation window, reduce endometrial receptivity, and lead to low clinical pregnancy rate.