Oligo(p-phenylenevinylene)-rhodium complex as intracellular catalyst for enhancing biosynthesis of polyhydroxybutyrate biomaterials

• 作者: Nan Dai Hao Zhao Wen Yu Fengting Lv Libing Liu Shu Wang
• 来源期刊: 中国科学：化学（英文版）
• 年，卷（期）: 2021,（1）
• 所属期刊栏目: ARTICLES
• 页码范围: 143-150
• 页数: 8页
• 语种: 英文

摘要

Microbial synthesis utilizes sustainable resources to produce valuable chemicals,as a potential alternative to petroleum-based chemical industry.Although metabolic engineering is an efficient method to enhance the biosynthesis efficacy of micro-organisms,it requires complicated biological procedures.Herein,we report a facile intracellular catalysis system for augmenting the production of bio-based material in microorganism.Covalent linking of oligo(p-phenylenevinylene)(OPV)and cyclo-pentadienyl rhodium(Ⅲ)bipyridine offers intracellular metal catalyst(OPV-Rh).The OPV-Rh complex displayed certain resistance to toxic biomolecules,which guaranteed its catalytic activity in complicated biological systems.With uptake by Gram-negative bacterium Ralstonia eutropha H16(R.eutropha H16),the OPV-Rh complex promotes the transformation of in-tracellular NADP+to NADPH,which further enhances the biosynthesis of polyhydroxybutyrate(PHB)by this microorganism.This work demonstrates that synthetic metal catalyst can be employed for regulating microbial biosynthesis intracellularly.