Feasibility study of 68Ga-labeled CAR T cells for in vivo tracking using micro-positron emission tomography imaging

• 作者: Xin-yu Wang Yan Wang Qiong Wu Jing-jing Liu Yu Liu Dong-hui Pan Wei Qi Li-zhen Wang Jun-jie Yan Yu-ping Xu Guang-ji Wang Li-yan Miao Lei Yu Min Yang
• 来源期刊: 中国药理学报（英文版）
• 年，卷（期）: 2021,（5）
• 所属期刊栏目: Chemotherapy
• 页码范围: 824-831
• 页数: 8页
• 语种: 英文

摘要

Clinical tracking of chimeric antigen receptor (CAR) T cells in vivo by positron emission tomography (PET) imaging is an area of intense interest.But the long-lived positron emitter-labeled CAR T cells stay in the liver and spleen for days or even weeks.Thus,the excessive absorbed effective dose becomes a major biosafety issue leading it difficult for clinical translation.In this studY we used 68Ga,a commercially available short-lived positron emitter,to label CAR T cells for noninvasive cell tracking in vivo.CAR T cells could be tracked in vivo by 68Ga-PET imaging for at least 6 h.We showed a significant correlation between the distribution of 89Zr and 68Ga-labeled CAR T cells in the same tissues (lungs,liver,and spleen).The distribution and homing behavior of CAR T cells at the early period is highly correlated with the long-term fate of CAR T cells in vivo.And the effective absorbed dose of 68Ga-labeled CAR T cells is only one twenty-fourth of 89Zr-labeled CAR T cells,which was safe for clinical translation.We conclude the feasibility of 68Ga instead of 89Zr directly labeling CAR T cells for noninvasive tracking of the cells in vivo at an early stage based on PET imaging.This method provides a potential solution to the emerging need for safe and practical PET tracer for cell tracking clinically.