Characterization and Optimization of Amylase Production in Strains LZ-10 and LZ-11 Belonging to Bacillus subtilis

• 作者: Zhao Shu-qin Yang Xiao-pu Gao Yuan
• 来源期刊: 东北农业大学学报（英文版）
• 学科: 生物学
• 年，卷（期）: 2022,（1）
• 页码范围: 79-88
• 页数: 10页
• 分类号: Q815
• 语种: 英文

摘要

In order to obtain pure enzyme with high activity, two amylase producing strains were isolated from soil samples, and named as the strains LZ-10 and LZ-11. According to morphologic observation, physiology and biochemistry experiments, 16S rRNA and gyrB gene analysis, the strains LZ-10 and LZ-11 were identified as Bacillus subtilis. Adopted the method of ammonium sulfate, DEAE-52 anion purify enzyme, finally used polyacrylamide gel electrophoresis (SDS-PAGE) to detect molecular weight. The strain LZ-10 had an amylase activity of 123.3 U ? mL-1, a purification factor of 6.8, a recovery rate of 69.5％ and an optimal temperature of 50℃. The amylase activity of the strain LZ-11 was 59.91 U ? mL-1, the purification factor was 4.5, the recovery rate was 60.5％, and the optimum temperature was 55℃. The commodity enzyme derived from Bacillus subtilis was 37.5 U ? mL-1. The relative molecular weight of amylase activity from the two strains was 55 ku. Both thermal stability and pH stability were higher than those of commercialized amylase.