Effect of pestle intervention in type 2 diabetic peripheral neuropathy on Keap1 / Nrf2 / ARE pathway and the relationship with oxidative stress

• 作者: Fang Wang Hui Yang Shun-Qi Liao Yao Wang Han Wang Xi-Mei Weng Ya-Ling Huang
• 来源期刊: 海南医科大学学报（英文版）
• 年，卷（期）: 2022,（6）
• 所属期刊栏目: Original article
• 页码范围: 24-28
• 页数: 5页
• 语种: 英文

摘要

Objective: To investigate the effect of pestle needle treatment on Nrf2 pathway and the relationship with oxidative stress in diabetic peripheral neuropathy. Methods: Patients with DPN who met the inclusion criteria were randomly divided into control and test groups with 30 patients in each group in a 1:1 allocation ratio. Both groups were given basic treatment, and the pestle group was treated with needle pestle therapy 5 times a week for a total of 4 weeks of intervention. Serum SOD and GSH PX levels were examined by colorimetry before and after intervention; Serum Keap1/Nrf2/ARE signaling pathway related factors expression levels were measured by ELISA; Keap1 and Nrf2 mRNA expression was determined by RT-PCR. Results: Compared with the control group, SOD and GSH-Px in the test group were significantly increased, Keap1 expression was decreased, Nrf2 expression was increased, Keap1 mRNA expression was significantly decreased, and Nrf2 mRNA expression was significantly increased. Conclusions: the pestle needle may enhance the body's antioxidant capacity by modulating the Keap1/Nrf2/ARE signaling pathway to enhance the production of its downstream antioxidant enzymes SOD and GSH Px, thereby protecting and repairing the damaged peripheral nerves in DPN patients.