基本信息来源于合作网站,原文需代理用户跳转至来源网站获取       
摘要:
AIM:To explore the possibility that PDX-1 gene is reactivated as a consequence of molecular events that occur during liver regeneration. METHODS:Rat hepatocytes were maintained in DMEM- F12,10%fetal bovine serum(FBS),penicillin/streptomycin and geneticin when applicable.Rat insulinoma RIN 1046-38 cells were maintained in M-199-10%FBS and penicillin/streptomycin.The final concentration of glucose was 11.1 mmol/L.During regeneration,lateral and medial liver lobes of adult male Wistar rats were surgically removed,with up 70%loss of liver mass.In methylation experiments,5-aza-deoxycytidine(5-aza-dC)was used.Primer3 software was used for poly- merase chain reaction(PCR).Quantitative real time PCR (qRT-PCR)was performed using SYBR Green technol- ogy;primers were designed by Beacon Designer 6 software.Western blotting and SDS-PAGE were performed according to standard procedures.Antibodies were purchased from commercial suppliers.RESULTS:We explored the possibility that liver regeneration could trigger PDX-1 expression,and hence insulin production.Twenty-four hours after surgical liver removal,regeneration was active as demonstrated by the increased proliferating cell nuclear antigen;however, all the other checked genes(involved in insulin gene expression):PC-1,Ngn3,NeuroD1,Btc,PDX-1 and Ins-1, were not related to the molecular events caused by this process.The only marker detected in regenerating liver was E47:a transcription factor of the the basic helixloop-helix family known to be expressed ubiquitously in mammalian cells.In the rat pancreas,almost all of the tested genes were expressed as shown by RT-PCR, except for Ngn3,which was silenced 2 d after birth. Therefore,the molecular events in liver regeneration are not sufficient to promote PDX-1 expression.DNA methylation is a known mechanism to achieve stable re- pression of gene expression in mammals:Hxk 2 gene is silenced through this mechanism in normal hepatocytes. The administration of 5-aza-dC to cultured cells is in fact able to upregulate Hxk 2 mRNA.We inves
推荐文章
腺相关病毒介导的pdx-1基因表达促进糖尿病大鼠肝脏细胞向胰岛素产生细胞转化的研究
腺相关病毒
胰十二指肠同源盒基因-1
胰岛素
糖尿病
高血糖
PDX-1基因克隆载体的体外构建
胰-十二指肠同源框-1
cDNA
胰岛细胞瘤
克隆载体
PDX-1蛋白对棕榈酸诱导的大鼠胰岛凋亡的保护作用
PDX-1蛋白
蛋白的表达和纯化
凋亡
棕榈酸
大鼠胰岛
内容分析
关键词云
关键词热度
相关文献总数  
(/次)
(/年)
文献信息
篇名 Regeneration and DNA demethylation do not trigger PDX-1 expression in rat hepatocytes
来源期刊 世界生物化学杂志:英文版(电子版) 学科 生物学
关键词 Transcription factor PDX-1 HEPATECTOMY Liver REGENERATION Quantitative real time POLYMERASE chain reaction DNA METHYLATION
年,卷(期) 2010,(9) 所属期刊栏目
研究方向 页码范围 281-285
页数 5页 分类号 Q78
字数 语种
DOI
五维指标
传播情况
(/次)
(/年)
引文网络
引文网络
二级参考文献  (0)
共引文献  (0)
参考文献  (1)
节点文献
引证文献  (0)
同被引文献  (0)
二级引证文献  (0)
2010(0)
  • 参考文献(0)
  • 二级参考文献(0)
  • 引证文献(0)
  • 二级引证文献(0)
研究主题发展历程
节点文献
Transcription
factor
PDX-1
HEPATECTOMY
Liver
REGENERATION
Quantitative
real
time
POLYMERASE
chain
reaction
DNA
METHYLATION
研究起点
研究来源
研究分支
研究去脉
引文网络交叉学科
相关学者/机构
期刊影响力
世界生物化学杂志:英文版(电子版)
季刊
1949-8454
北京市朝阳区东四环中路62号楼远洋国际中
出版文献量(篇)
391
总下载数(次)
0
  • 期刊分类
  • 期刊(年)
  • 期刊(期)
  • 期刊推荐
论文1v1指导