Effect of deforolimus and VEGF on angiogenesis in endometrial stromal cells following three-dimensional culture

• 作者: Armin Ai Jafar Ai Naghmeh Bahrami Roya Karimi Somayeh Ebrahimi
• 来源期刊: 干细胞探索(英文)
• 学科: 医学
• 关键词: ENDOMETRIAL STROMAL Cells ENDOMETRIUM 3-D culture Deforolimus VEGF
• 年，卷（期）: 2013,（1）
• 页码范围: 7-12
• 页数: 6页
• 分类号: R73

摘要

The presence of endometrial tissue outside of the uterine cavity is named endometriosis and is the most common gynecologic disorder in women. Determining the inhibitory effect of a Deforolimus on angiogenesis in a three-dimensional (3-D) culture of human endometrial stromal cells (hEnCs) in vitro. The important mechanism in the pathogenesis of endometriosis is angiogenesis, and deforolimus has been shown to have anti-angiogenic activity. This was an in vitro study of human endometrial stromal cells in 3-D culture of fibrin matrix. Endometrial stromal cells isolated and placed in a 3-D fibrin matrix culture system for angiogenesis with VEGF and inhibit angiogenesis by deforolimus. Finally these cells analyzed by CD31 antibodies. After 3 weeks, in cells treated with VEGF, endothelial cell branching was observed and rudimentary capillary-like structures formed. In the presence of 5μM of deforolimus, angiogenesis was reduced. The deforolimus were shown to be effective in inhibiting the mechanisms of angiogenesis.