Inhibition Mechanism of Antifungal Monomer DZP8 against Rhizoctonia solani

• 作者: Feng Huagen Lu Hui Tu Guoquan Tu Xiaorong Wen Meiping Xu Jia
• 来源期刊: Plant Diseases and Pests(植物病虫害研究：英文版)
• 学科: 农学
• 关键词: 水稻纹枯病菌 抗真菌药物 抑制机制 单体 菌丝体培养液 细胞膜损伤 菌丝形态 链霉菌702
• 年，卷（期）: 2013,（1）
• 页码范围: 18-22
• 页数: 5页
• 分类号: S435.111.4

摘要

In order to study the inhibition mechanism of antifungal monomers DZP8 produced by streptomyces 702 strain against Rhizoctonia solani, the effects of DZP8 on mycelial morphology were investigated under light microscope, and the effects of DZP8 on mycelial inclusion leakage and cell membrane damage of mycelium were determined. The results showed that DZP8 caused a series of changes in mycelial morphology of R. solani in liquid culture condition. DZP8 treatments with concentrations of 1.81 and 3.35 μg/mL for 24 h caused big vacuole, rough surface and more inclusions of mycelium. With the treatment time prolonging, the mycelium distorted and appeared irregular constriction. DZP8 treatment with concentration of 20.10 μg/mL led to the increase of conductivity of mycelium culture liquid, leakage of soluble sugar and protein, and the lipid peroxidation of mycelium membrane. It was found that DZP8 at a very low concentration could cause changes of mycelial morphology of R. solani, while only a certain concentration could cause significant damage to cell membrane. This indicated that cell membrane might be one of the action sites of DZP8, and it might have other action sites or mechanism.