Evidence for Tumour Suppressor Function of DOK7 in Human Breast Cancer

• 作者: Abdul Kasem James Bracken Kefah Mokbel Tamara Ghanem Wen G. Jiang
• 来源期刊: 癌症治疗(英文)
• 学科: 医学
• 关键词: Breast Cancer DOK-7 CPG HYPERMETHYLATION TUMOUR SUPPRESSOR PROGNOSTIC Marker
• 年，卷（期）: 2014,（1）
• 页码范围: 67-73
• 页数: 7页
• 分类号: R73

摘要

Introduction: Downstream of tyrosine kinase 7 (DOK-7) is a member of the DOK family, which has been associated with the development and progression of various humancancers. Previously, identification of CpG hypermethylation in DOK-7 promoter was identified in breast cancer. Method: DOK-7 mRNA extraction and reverse transcription were performed on fresh frozen breast cancer tissue samples and normal background breast tissue. Transcript levels of expression were analyzed against TNM stage, tumour grade and clinical outcome over a 10-year follow-up period. Results: Levels of DOK-7 expression decreased significantly with increasing TNM stage. Higher DOK-7 expression was correlated with longer disease free and overall survival times. Conclusion: To our knowledge, this is the first study to investigate DOK-7 expression in human breast cancer. We identify a potential DOK-7 tumour suppressor role. DOK-7 as a prognostic biomarker in human breast cancer should be included in future validation studies.