Establishment of Cytokines Quantitative PCR Methods and Its Application in Anaphylaxis Study of Houttuynia Cordata Injection

• 作者: Fengjiao Sun Li Li Wen Zeng Xiangqi Zeng Xiaolu Cheng
• 来源期刊: 生物科学与医学(英文)
• 学科: 医学
• 关键词: CYTOKINES QUANTITATIVE PCR ANAPHYLAXIS Houttuynia Cordata INJECTION
• 年，卷（期）: 2015,（7）
• 页码范围: 1-8
• 页数: 8页
• 分类号: R73

摘要

We design specific primers, according to the four kinds of important macaque cytokines, based on macaque β-action genes, then extract total RNA in peripheral blood mononuclear cells of macaques, and trascriptase cDNA reversely after establishing real-time quantitative PCR, and test the relative expression amount of several kinds of important cytokines during the hypersensitive process. The testing method of quantitative PCR in this experiment is highly sensitive, all cytokines are up to 102 copies/μL and highly reproducible, the intraassay and interassay coefficients of variation are less than 6%. The method applied to the sensitive reaction experiment on cytokines induced by houttuynia cordata turns out that the cytokines relative expression of houttuynia cordata distillate with low dose group and high dose group is significantly higher than that of saline group (P > 0.01). Meanwhile, significantly increased IL-4, IL-10 and IL-13 and decreased IFN-γ are found in both group A and group B after houttuynia injection (P > 0.05).