Genetic Relationships of Soft Rot Bacteria Isolated from Konjac in China by Amplified Fragment Length Polymorphism (AFLP) and 16S rDNA Gene Sequences

• 作者: Chaozhu Yang Jinping Wu Zhenbiao Jiao Zhengming Qiu Zhongli Hu Zili Ding
• 来源期刊: 农业科学(英文)
• 学科: 农学
• 关键词: SOFT ROT BACTERIA Identification 16S rDNA AFLP Genetic Diversity
• 年，卷（期）: 2015,（7）
• 页码范围: 717-723
• 页数: 7页
• 分类号: S5

摘要

Twenty-three isolates of soft rot bacteria from konjac corms were examined for their diversity using 16S rDNAs and AFLP technology. Both methods clustered two groups, dependent on their biotype characterization of Pectobacterium carotovora subsp. carotovora (P.c.c) and Pectobacterium chrysanthemi (P.ch), respectively. Of all isolates, 17 (73.9%) belonged to P. ch, indicated as the main pathogenic bacteria of konjac producing areas in China. The genetic variation among isolates from the same biotype was also rich, not consistent with the distances of the geographic sources.