Evaluation of biodegradable electric conductive tube-guidesand mesenchymal stem cells

• 作者: Jorge Ribeiro, Tiago Pereira, Ana Rita Caseiro, Paulo Armada-da-Silva, Isabel Pires, Justina Prada, Irina Amorim, Sandra Amado, Miguel Fran #x000e7 a, Carolina Gon alves, Maria Ascens #x000e3 o Lopes, Jos #x000e9 Domingos Santos, Dina Morais Silva, Stefano Geuna, Ana L #x000fa cia Lu #x000ed s, and Ana Colette Maur cio
• 来源期刊: 世界干细胞杂志：英文版（电子版）
• 学科: 医学
• 关键词: Nerve regeneration Neurotmesis Tubeguides MESENCHYMAL stem cells Carbon NANOTUBES Functional assessment GAIT BIOMECHANICAL analysis Histomorfometry Polyvinyl alcohol
• 年，卷（期）: 2015,（6）
• 页码范围: 956-975
• 页数: 20页
• 分类号: R

摘要

AIM To study the therapeutic effect of three tubeguideswith electrical conductivity associated tomesenchymal stem cells （MSCs） on neuro-muscularregeneration after neurotmesis.METHODS： Rats with 10-mm gap nerve injury weretested using polyvinyl alcohol （PVA）, PVA-carbonnanotubes （CNTs） and MSCs, and PVA-polypyrrole（PPy）. The regenerated nerves and tibialis anteriormuscles were processed for stereological studies after20 wk. The functional recovery was assessed serially forgait biomechanical analysis, by extensor postural thrust,sciatic functional index and static sciatic functional index （SSI）, and by withdrawal reflex latency （WRL）. Invitro studies included cytocompatibility, flow cytometry,reverse transcriptase polymerase chain reaction andkaryotype analysis of the MSCs. Histopathology of lung,liver, kidneys, and regional lymph nodes ensured thebiomaterials biocompatibility.RESULTS： SSI remained negative throughout andindependently from treatment. Differences betweentreted groups in the severity of changes in WRL existed,showing a faster regeneration for PVA-CNTs-MSCs （P〈 0.05）. At toe-off, less acute ankle joint angles wereseen for PVA-CNTs-MSCs group （P = 0.051） suggestingimproved ankle muscles function during the push offphase of the gait cycle. In PVA-PPy and PVA-CNTsgroups, there was a 25% and 42% increase of averagefiber area and a 13% and 21% increase of the ＂minimalFeret＇s diameter＂ respectively. Stereological analysisdisclosed a significantly （P 〈 0.05） increased myelinthickness （M）, ratio myelin thickness/axon diameter （M/d） and ratio axon diameter/fiber diameter （d/D; g-ratio）in PVA-CNT-MSCs group （P 〈 0.05）.CONCLUSION： Results revealed that treatmentwith MSCs and PVA-CNTs tube-guides induced betternerve fiber regeneration. Functional and kinematicsanalysis revealed positive synergistic effects brought byMSCs and PVA-CNTs. The PVA-CNTs and PVA-PPy arepromising scaffolds with electric conductive properties,bio- and cytocompatible tha