Effects of 530 nm monochromatic light on basic fibroblast growth factor and transforming growth factor-β1 expression in Müller cells

• 作者: Jing Xia Lei Zhong Wei Xia Xiao-Feng Zhang Xin-Yi Fu Ying-Ming Wang Zheng-Tai Sun
• 来源期刊: 国际眼科杂志：英文版
• 学科: 医学
• 关键词: 单色的光 近视 Mü ller 房间 基本成纤维细胞生长因素 转变生长 factor-β 1
• 年，卷（期）: 2015,（5）
• 页码范围: 904-909
• 页数: 0页
• 分类号: R329.2

摘要

AIM: To expose rat retinal Müller cells to 530 nm monochromatic light and investigate the influence of varying light illumination times on basic fibroblast growth factor(b FGF) and transforming growth factor-β1(TGF-β1) expression.·METHODS: Three groups of rat retinal Müller cells cultured in vitro under a 530 nm monochromatic light were divided into 6, 12 and 24 h experimental groups,while cells incubated under dark conditions served as the control group. The b FGF and TGF-β1 m RNA expression, protein levels and fluorescence intensity of the Müller cells were analyzed.·RESULTS: The b FGF m RNA expression and protein levels were significantly upregulated in Müller cells in all three experimental groups compared with the control group(P <0.05), while that of TGF-β1 was downregulated(P <0.05). Also, b FGF expression was positively correlated,but TGF-β1 expression was negatively correlated with illumination time. The largest changes for both cytokines were seen in the 24 h group. The changes in b FGF and TGF-β1 fluorescence intensity were highest in the 24 h group, and significant differences were observed among the experimental groups(P <0.05).·CONCLUSION: The expressions of b FGF and TGF-β1changed in a time-dependent manner in Müller cells exposed to 530 nm monochromatic light with 250 lx illumination intensity. Müller cells might play a role in the development of myopia by increasing b FGF expression or decreasing TGF-β1 expression. Changes in cytokine expression in retinal Müller cells may affect monochromatic light-induced myopia.