感染“中四”小麦条锈菌T4菌系SCAR检测标记的开发

• 作者: 康振生 李强 李高宝 樊玉 王保通 郭婧
• 来源期刊: 植物病理学报
• 学科: 农学
• 年，卷（期）: 2017,（2）
• 所属期刊栏目: 研究简报
• 页码范围: 270-273
• 页数: 4页
• 分类号: S435.121.42
• 语种: 中文
• DOI: 10.13926/j.cnki.apps.000043

摘要

Stripe rust,caused by Puccinia striiformis f.sp.tritici (Pst),is one of the most important diseases on wheat in China.Zhong 4,a partial amphiploid (2n =56) carrying resistance from Thinopyrum intermedium and resistant to most of Pst pathotypes identified so far.T4 is one of the very rare Pst pathotypes which are virulent to Zhong 4.With the aim of developing race-specific molecular markers for detection of Pst pathotype T4,214 random amplified polymorphic DNA (RAPD) primers were used to test polymorphism among pathotype T4 and six predominant pathotypes CYR29,CYR31,CYR32,CYR33,Su11-4 and V26.An unique 821 bp band was identified in T4 with primer S1363.The band was cloned and sequenced.Based on the sequences,sequence characterized amplified region(SCAR) marker T4sp1/sp2 was developed to differentiate T4.The SCAR marker was then validated with DNA samples prepared from leaves of wheat cultivar Mingxian 169 after inoculated with T4,CYR29,CYR31,CYR32,CYR33,Su11-4,V26 and water (mock-inoculated).The specific band was produced only in samples prepared from plants after inoculated with T4 six days.Therefore,the SCAR marker T4sp1/sp2 can specifically detect T4.

作者信息

序号	姓名	单位	发文数	被引次数	H指数	G指数
1	康振生		142	2538	28.0	45.0
2	李强		63	357	11.0	15.0
3	王保通		35	266	10.0	14.0
4	李高宝		13	62	5.0	7.0
5	郭婧		2	12	1.0	2.0
6	樊玉		1	0	0.0	0.0