An investigation of the possible methods and potential benefits of de novo cloning of Nannochloropsis oceanica genes

• 作者: Guanpin Yang Hang Liu Li Guo Zhongyi Zhang
• 来源期刊: 海洋生命科学与技术(英文)
• 学科: 生物学
• 关键词: NANNOCHLOROPSIS oceanica Bulked MUTANT analysis MUTANT library De novo CLONING REVERSE GENETICS
• 年，卷（期）: 2019,（1）
• 页码范围: 22-27
• 页数: 6页
• 分类号: Q78

摘要

Species in the microalgal genus Nannochloropsis are increasingly used as models for theoretical and applied studies. Herewe attempt to generate InDei variations in the genome of Nannochloropsis oceanica, and then decipher the genetic basisof its economic and biological traits with bulked mutant analysis modified from bulked segregant analysis. In addition, wedescribe our efforts to construct site-tagged and gene-traceable mutant libraries to clone its genes through reverse geneticapproaches. Currently, more than a half of N. oceanica protein-encoding genes are annotated against databanks. However, nofunctional gene has been de novo cloned from N. oceanica and no new function has been assigned to any of its annotatablegenes. Here, we discuss the possible methods and potential benefits of de novo cloning of N. oceanica genes.