Cloning and Analysis of Chitinase Gene in Bacillus subtilis HAS

• 作者: Cai Wenwei Wang Jungang Xiong Guoru Yang Benpeng Zhang Shuzhen Zhao Gengfeng
• 来源期刊: Plant Diseases and Pests(植物病虫害研究:英文版)
• 学科: 农学
• 关键词: BACILLUS SUBTILIS HAS Sporisorium scitaminea Syd. ANTIFUNGAL protein CHITINASE gene
• 年，卷（期）: 2019,（2）
• 页码范围: 4-6
• 页数: 3页
• 分类号: S

摘要

[Objective] The paper was to study the possible mechanism of chitinase gene.[Method] The chitinase gene with inhibitory effect against a variety of pathogenic fungi was cloned and analyzed in this study.[Result] The genome of HAS strain contained an ORF of 834 bp. The homology of coding protein corresponding to ORF with chitosanase [Bacillus subtilis subsp. subtilis str. 168](ref|NP_390566.1) was the highest of 98%(272/277).[Conclusion]Through the analysis of amino acid sequence and secondary structure of the protease, tertiary structure prediction and analysis, HAS strain may play a pivotal role in controlling Sporisorium scitaminea Syd.