Virus-induced Phytoene Desaturase (PDS) Gene Silencing Using Tobacco Rattle Virus in Lilium × formolongi

• 作者: Guoren He Hua Xu Jingyi Lei Jun Ming Leifeng Xu Panpan Yang Suxia Yuan Yuchao Tang Yuwei Cao
• 来源期刊: 园艺学报（英文版）
• 年，卷（期）: 2019,（1）
• 所属期刊栏目: Research Papers
• 页码范围: 31-38
• 页数: 8页
• 语种: 英文

摘要

Gene function analysis is challenging for Lilium due to the lack of an efficient method for stable genetic transformation. Virus-induced gene silencing (VIGS) is an attractive tool for determining gene function in plants. This study reported that Tobacco rattle virus (TRV)-based VIGS of a PHYTOENE DESATURASE (PDS) ortholog (LhPDS) can be achieved in Lilium × formolongi seedlings, with a survival rate of 92％, using the inoculation method of rubbing plus injection. Compared with untreated and mock-treated seedlings, the photobleached leaf phenotype of silenced plants significantly correlates with down-regulation of endogenous LhPDS (P ≤ 0.05). In addition, the silencing phenomenon can be observed in the growing points of plants, indicating that systemic viral infection was achieved using the protocol. The results indicate that TRV-based VIGS can be used to characterize gene function in Lilium × formolongi. This work lays the foundation for gene function analysis and molecular breeding in Lilium spp.

作者信息

序号	姓名	单位	发文数	被引次数	H指数	G指数
1	Hua Xu	中国农业科学院蔬菜花卉研究所	1	0	0.0	0.0
2	Leifeng Xu	中国农业科学院蔬菜花卉研究所	1	0	0.0	0.0
3	Panpan Yang	中国农业科学院蔬菜花卉研究所	1	0	0.0	0.0
4	Yuwei Cao	中国农业科学院蔬菜花卉研究所	1	0	0.0	0.0
5	Yuchao Tang	中国农业科学院蔬菜花卉研究所	1	0	0.0	0.0
6	Guoren He	中国农业科学院蔬菜花卉研究所	1	0	0.0	0.0
7	Suxia Yuan	中国农业科学院蔬菜花卉研究所	1	0	0.0	0.0
8	Jingyi Lei	中国农业科学院蔬菜花卉研究所	1	0	0.0	0.0
9	Jun Ming	中国农业科学院蔬菜花卉研究所	1	0	0.0	0.0