Recent advances in structural studies of the CRISPR-Cas-mediated genome editing tools

• 来源期刊: 国家科学评论（英文版）
• 年，卷（期）: 2019,（3）
• 页码范围: 438-451
• 页数: 14页
• 语种: 英文
• DOI: 10.1093/nsr/nwy150

摘要

Clustered regularly interspaced short palindromic repeats (CRISPR) and accompanying CRISPR-associated (Cas) proteins provide RNA-guided adaptive immunity for prokaryotes to defend themselves against viruses.The CRISPR-Cas systems have attracted much attention in recent years for their power in aiding the development ofgenome editing tools.Based on the composition of the CRISPR RNA-effector complex,the CRISPR-Cas systems can be divided into two classes and six types.In this review,we summarize recent advances in the structural biology of the CRISPR-Cas-mediated genome editing tools,which helps us to understand the mechanism of how the guide RNAs assemble with diverse Cas proteins to cleave target nucleic acids.