Validation for Microbial Limit Test Method of Compound Yu E Nose Drops

• 作者: Peixue CAO Renhui YANG Xiangling QU Zhen ZENG
• 来源期刊: 农业生物技术:英文版
• 学科: 农学
• 关键词: COMPOUND Yu E NOSE DROPS MICROBIAL LIMIT test DILUTION method
• 年，卷（期）: 2019,（5）
• 页码范围: 125-128
• 页数: 4页
• 分类号: S

摘要

[Objectives] This study was conducted to establish a method for microbial limit test of Compound Yu E Nose Drops.[Methods] According to the Chinese Pharmacopoei (General Rules in the fourth part of the 2015 edition),the microbial limit test method for Compound Yu E Nose Drops was verified.[Results] Compound Yu E Nose Drops has a strong inhibitory effect on Staphylococcus aureus and Bacillus subtilis,and the inhibitory activity was significantly eliminated after increasing the diluent (1∶ 20).The recoveries were all in the range of 0.5-2.0 when the total quantities of aerobic microbes were determined by the dilution method (1∶ 20).When the total quantities of mould and yeast were determined by the conventional method,the recoveries were both in the range of 0.5-2.0.When examining control bacteria,Escherichia coli,S.aureus and Pseudomonas aeruginosa can all be detected in the test groups by the test liquid dilution method.[Conclusions] For Compound Yu E Nose Drops,the total quantities of aerobic microbe can be counted by the dilution method;the quantities of mould and yeast can be examined by the conventional plate method;and the conventional method can be used for control microbe examination.