Delivery methods for CRISPR/Cas9 gene editing in crustaceans

• 作者: Sen Xu Swatantra Neupane Thinh Phu Pham
• 来源期刊: 海洋生命科学与技术(英文)
• 学科: 生物学
• 关键词: MICROINJECTION ELECTROPORATION VITELLOGENESIS DAPHNIA Parhyale hawaiensis Exopalaemon carinicauda
• 年，卷（期）: 2020,（1）
• 页码范围: 1-5
• 页数: 5页
• 分类号: Q78

摘要

In this mini-review,we provide an up-to-date overview of the delivery methods that have been used for CRISPR/Cas9 genomic editing in crustacean species.With embryonic microinjection as the main workforce for delivering CRISPR/Cas9 reagents,biologists working with crustacean species have to tackle the technical challenges involved in microinjection.We use examples of three Crustacean species(the branchiopod Daphnia,amphipod Parhyale hawaiensis,and decapod Exopalaemon carinicauda)to provide a technical guide for embryonic microinjection.Moreover,we outline two potentially useful new techniques for delivering CRISPR/Cas9 components into crustaceans,i.e.,Receptor-Mediated Ovary Transduction of Cargo(ReMOT Control)and electroporation.