Study on Relationship Between Differential Proteins of Bacillus cereus LBR-4 and Its Salt Tolerance Mechanism

• 作者: Du Chuan-ying Gao Ji-guo Li Hai-tao Ni He-jia Tan Jia-li Wang Jian
• 来源期刊: 东北农业大学学报（英文版）
• 学科: 工学
• 年，卷（期）: 2020,（3）
• 页码范围: 53-62
• 页数: 10页
• 分类号: TS201.3
• 语种: 英文

摘要

In order to explore the salt tolerance mechanism of Bacillus cereus LBR-4 with salinity of 14％ NaCl, differential proteomic analysis of the whole protein of LBR-4 strain expressed under 14％ NaCl high salinity condition and normal culture condition (1％ NaCl) was studied by two-dimensional electrophoresis and mass spectrometry. The isoelectric point of most detected proteins was between pH 4-7 and the molecular weight distribution was 10-70 ku. Compared with the normal culture condition, the expression level of 118 protein spots in the whole protein expression map changed significantly (accounting for 25.2％ of the total protein spots). The expression level of 78 protein spots increased significantly, including 22 new protein spots that appeared under high salt stress. The expression levels of 40 protein spots decreased significantly, including 18 protein spots that disappeared under high salt stress. By mass spectrometry, six distinct differentially expressed protein spots were dihydroxy acid dehydratase, cell division protein FtsZ, iron sulfur cluster synthesis protein SufD, unknown carboxylase YngE, hypothetical acetaldehyde dehydrogenase DhaS and phenylalanine acid tRNA ligase alpha subunit. It was speculated that under high salt stress, the cells had protective measures and the secretion of intracellular compatible solutes increased. The iron and sulfur clusters involved in various physiological reactions also activated the stressful suf synthesis pathway, and the rate of cell division and reproduction was also slowed down and ensured the normal progress of physiological reactions in the cells.

作者信息

序号	姓名	单位	发文数	被引次数	H指数	G指数
1	Tan Jia-li		1	0	0.0	0.0
2	Du Chuan-ying		1	0	0.0	0.0
3	Wang Jian		1	0	0.0	0.0
4	Ni He-jia		1	0	0.0	0.0
5	Gao Ji-guo		1	0	0.0	0.0
6	Li Hai-tao		1	0	0.0	0.0