Construction of Technology System on Development and Repropagation in Vitro of Several Cultivars in Pear

• 作者: Baofeng HAO Haie ZHANG Jicheng HAN Jie LI Jintao XU Lijuan GAO Longfei LI Minghui JI Shujun ZHAO Yu WANG
• 来源期刊: 农业生物技术:英文版
• 学科: 农学
• 关键词: PEAR Tissue culture Development and propagation technology system
• 年，卷（期）: 2020,（4）
• 页码范围: 12-18
• 页数: 7页
• 分类号: S51

摘要

It is the most available way to obtain self-rooted seedlings by tissue culture,which could breed de-virus seedlings with uniform traits and contribute to improve seedling quality.To construct a complete and efficient technology system,this test used stem with buds of 16 cultivars and super lines including Yali,Jingbaili,03-04-034,03-08-080 and so on as materials to study the best way for explant sampling time and position,sterilization time using HgCl 2 and hormone concentrations.The result indicated that the optimum technology system was that the middle parts of longest shoots before 20 d after full-blossom were sterilized for 8 min by 0.1%HgCl 2 and inoculated on the development medium MS+6-BA 0.5 mg/L+IBA 0.2 mg/L+saccharose 3%+agar 0.6%(pH=5.8),and then trans-inoculated into propagation medium MS+6-BA 1.0 mg/L+IBA 0.3 mg/L+saccharose 3%+agar 0.6%(pH=5.8).