Targeted mutagenesis in Arabidopsis thaliana using CRISPR-Cas12b/C2c1

• 作者: Dejing Kong Fan Wu Haokun Du Langyu Liu Lingfeng Shi Lulu Wang Quan Wang Xinyu Qiao Ya-Jie Zhang Yafei Zhao Yifan Gao Yu Zhang Ziyi Zhang
• 来源期刊: 植物学报（英文版）
• 年，卷（期）: 2020,（11）
• 所属期刊栏目: Breakthrough Report
• 页码范围: 1653-1658
• 页数: 6页
• 语种: 英文
• DOI: 10.1111/jipb.12944

摘要

Cas12b/C2c1 is a newly identified class 2 CRISPR endonuclease that was recently engineered for targeted genome editing in mammals and rice. To explore the po-tential applications of the CRISPR-Cas12b system in the dicot Arabidopsis thaliana, we selected BvCas12b and BhCas12b v4 for analysis. We successfully used both endonucleases to induce mutations, perform multiplex genome editing, and create large deletions at multiple loci. No significant mutations were detected at potential off-target sites. Analysis of the insertion/deletion frequen-cies and patterns of mutants generated via targeted gene mutagenesis highlighted the potential utility of CRISPR-Cas12b systems for genome editing in Arabidopsis.